Detailed information of TA system TA042651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 48064..48599
• Replicon: plasmid pNUITMVRE1_1
• Accession: NZ_AP026774
• Organism: Enterococcus faecium strain NUITM-VRE1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: R2PQC0
• Locus tag: OO969_RS14610
• Protein ID: WP_002314366.1
• Coordinates: 48064..48336 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q3Y220
• Locus tag: OO969_RS14615
• Protein ID: WP_002297422.1
• Coordinates: 48336..48599 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO969_RS14570 (NUITMVRE1_28650)	43166..43615	-	450	WP_010729386.1	TlpA disulfide reductase family protein	-
OO969_RS14575 (NUITMVRE1_28660)	43771..44313	-	543	WP_002298088.1	hypothetical protein	-
OO969_RS14580	44588..45231	+	644	Protein_53	transposase	-
OO969_RS14585 (NUITMVRE1_28700)	45307..45991	+	685	Protein_54	IS6-like element IS1216 family transposase	-
OO969_RS14590 (NUITMVRE1_28710)	46050..46238	-	189	Protein_55	hypothetical protein	-
OO969_RS14595 (NUITMVRE1_28720)	46239..47096	-	858	WP_002292680.1	ParA family protein	-
OO969_RS14600 (NUITMVRE1_28730)	47382..47668	-	287	Protein_57	type II toxin-antitoxin system RelE/ParE family toxin	-
OO969_RS14605 (NUITMVRE1_28740)	47658..47987	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
OO969_RS14610 (NUITMVRE1_28750)	48064..48336	-	273	WP_002314366.1	Txe/YoeB family addiction module toxin	Toxin
OO969_RS14615 (NUITMVRE1_28760)	48336..48599	-	264	WP_002297422.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
OO969_RS14620 (NUITMVRE1_28770)	48836..49240	+	405	WP_002287522.1	IS200/IS605-like element ISEfa4 family transposase	-
OO969_RS14625 (NUITMVRE1_28790)	49257..50404	+	1148	Protein_62	IS200/IS605 family element RNA-guided endonuclease TnpB	-
OO969_RS14630 (NUITMVRE1_28810)	50723..51631	+	909	Protein_63	IS982-like element ISEfm1 family transposase	-
OO969_RS14635 (NUITMVRE1_28820)	51634..52809	-	1176	WP_002336205.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..235911	235911
	-	inside	IScluster/Tn	-	-	36498..61173	24675

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10780.24 Da
• Isoelectric Point: 9.9724

>T42651 WP_002314366.1 NZ_AP026774:c48336-48064 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T42651 NZ_AP026774:c48336-48064 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9959.22 Da
• Isoelectric Point: 4.5370

>AT42651 WP_002297422.1 NZ_AP026774:c48599-48336 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT42651 NZ_AP026774:c48599-48336 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A829F704

Antitoxin

• AlphaFold DB: A0A829F006