Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 150434..150969 Replicon plasmid pGK961_1
Accession NZ_AP026667
Organism Enterococcus faecium strain GK961

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag OQH70_RS15200 Protein ID WP_002305108.1
Coordinates 150434..150706 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag OQH70_RS15205 Protein ID WP_002303154.1
Coordinates 150706..150969 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH70_RS15170 (EfmGK961_29830) 145448..146586 - 1139 Protein_148 NADP-dependent malic enzyme -
OQH70_RS15175 (EfmGK961_29840) 146814..147790 + 977 Protein_149 DNA-binding transcriptional regulator -
OQH70_RS15180 (EfmGK961_29850) 148059..148607 - 549 WP_002292681.1 hypothetical protein -
OQH70_RS15185 (EfmGK961_29860) 148608..149465 - 858 WP_002292680.1 ParA family protein -
OQH70_RS15190 (EfmGK961_29870) 149751..150038 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
OQH70_RS15195 (EfmGK961_29880) 150028..150357 - 330 WP_002292678.1 AbrB family transcriptional regulator -
OQH70_RS15200 (EfmGK961_29890) 150434..150706 - 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
OQH70_RS15205 (EfmGK961_29900) 150706..150969 - 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
OQH70_RS15210 (EfmGK961_29910) 151075..151398 - 324 WP_224453712.1 hypothetical protein -
OQH70_RS15215 (EfmGK961_29920) 151497..152060 - 564 Protein_157 IS6-like element ISS1N family transposase -
OQH70_RS15220 (EfmGK961_29940) 152140..153302 + 1163 WP_102642289.1 IS3 family transposase -
OQH70_RS15225 (EfmGK961_29950) 153774..154673 - 900 WP_002303235.1 aldose 1-epimerase family protein -
OQH70_RS15230 (EfmGK961_29960) 154694..154966 - 273 WP_002305107.1 DUF3884 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..173272 173272


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T42578 WP_002305108.1 NZ_AP026667:c150706-150434 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T42578 NZ_AP026667:c150706-150434 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT42578 WP_002303154.1 NZ_AP026667:c150969-150706 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT42578 NZ_AP026667:c150969-150706 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References