Detailed information of TA system TA042573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 126514..127049
• Replicon: plasmid pGK941_1
• Accession: NZ_AP026661
• Organism: Enterococcus faecium strain GK941

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q3Y221
• Locus tag: OQH75_RS14945
• Protein ID: WP_002305108.1
• Coordinates: 126514..126786 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A133CED7
• Locus tag: OQH75_RS14950
• Protein ID: WP_002303154.1
• Coordinates: 126786..127049 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OQH75_RS14915 (EfmGK941_29570)	121521..122708	-	1188	WP_002303156.1	NADP-dependent malic enzyme	-
OQH75_RS14920	122890..123842	+	953	Protein_121	DNA-binding transcriptional regulator	-
OQH75_RS14925 (EfmGK941_29600)	124139..124687	-	549	WP_002292681.1	hypothetical protein	-
OQH75_RS14930 (EfmGK941_29610)	124688..125545	-	858	WP_002292680.1	ParA family protein	-
OQH75_RS14935 (EfmGK941_29620)	125831..126118	-	288	WP_002300557.1	type II toxin-antitoxin system RelE/ParE family toxin	-
OQH75_RS14940 (EfmGK941_29630)	126108..126437	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
OQH75_RS14945 (EfmGK941_29640)	126514..126786	-	273	WP_002305108.1	Txe/YoeB family addiction module toxin	Toxin
OQH75_RS14950 (EfmGK941_29650)	126786..127049	-	264	WP_002303154.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
OQH75_RS14955 (EfmGK941_29660)	127155..127478	-	324	WP_224453712.1	hypothetical protein	-
OQH75_RS14960 (EfmGK941_29670)	127577..128140	-	564	Protein_129	IS6-like element ISS1N family transposase	-
OQH75_RS14965 (EfmGK941_29690)	128220..129381	+	1162	Protein_130	IS3 family transposase	-
OQH75_RS14970 (EfmGK941_29700)	129853..130752	-	900	WP_002303235.1	aldose 1-epimerase family protein	-
OQH75_RS14975 (EfmGK941_29710)	130773..131045	-	273	WP_002305107.1	DUF3884 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..173397	173397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10806.28 Da
• Isoelectric Point: 9.9080

>T42573 WP_002305108.1 NZ_AP026661:c126786-126514 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T42573 NZ_AP026661:c126786-126514 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9886.16 Da
• Isoelectric Point: 4.5370

>AT42573 WP_002303154.1 NZ_AP026661:c127049-126786 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT42573 NZ_AP026661:c127049-126786 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A133CER3

Antitoxin

• AlphaFold DB: A0A133CED7