Detailed information of TA system TA042568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 85906..86441
• Replicon: plasmid pGK923_1
• Accession: NZ_AP026656
• Organism: Enterococcus faecium strain GK923

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q3Y221
• Locus tag: OQH82_RS14725
• Protein ID: WP_002305108.1
• Coordinates: 86169..86441 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A133CED7
• Locus tag: OQH82_RS14720
• Protein ID: WP_002303154.1
• Coordinates: 85906..86169 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OQH82_RS14695 (EfmGK923_28930)	81910..82182	+	273	WP_002305107.1	DUF3884 family protein	-
OQH82_RS14700 (EfmGK923_28940)	82203..83102	+	900	WP_002303235.1	aldose 1-epimerase family protein	-
OQH82_RS14705 (EfmGK923_28950)	83574..84735	-	1162	Protein_91	IS3 family transposase	-
OQH82_RS14710 (EfmGK923_28970)	84815..85378	+	564	Protein_92	IS6-like element ISS1N family transposase	-
OQH82_RS14715 (EfmGK923_28980)	85477..85800	+	324	WP_224453712.1	hypothetical protein	-
OQH82_RS14720 (EfmGK923_28990)	85906..86169	+	264	WP_002303154.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
OQH82_RS14725 (EfmGK923_29000)	86169..86441	+	273	WP_002305108.1	Txe/YoeB family addiction module toxin	Toxin
OQH82_RS14730 (EfmGK923_29010)	86518..86847	+	330	WP_002292678.1	AbrB family transcriptional regulator	-
OQH82_RS14735 (EfmGK923_29020)	86837..87124	+	288	WP_002300557.1	type II toxin-antitoxin system RelE/ParE family toxin	-
OQH82_RS14740 (EfmGK923_29030)	87410..88267	+	858	WP_002292680.1	ParA family protein	-
OQH82_RS14745 (EfmGK923_29040)	88268..88816	+	549	WP_002292681.1	hypothetical protein	-
OQH82_RS14750 (EfmGK923_29050)	89113..90060	-	948	WP_265169954.1	sugar-binding domain-containing protein	-
OQH82_RS14755 (EfmGK923_29060)	90245..91432	+	1188	WP_002303156.1	NADP-dependent malic enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..172275	172275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10806.28 Da
• Isoelectric Point: 9.9080

>T42568 WP_002305108.1 NZ_AP026656:86169-86441 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T42568 NZ_AP026656:86169-86441 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9886.16 Da
• Isoelectric Point: 4.5370

>AT42568 WP_002303154.1 NZ_AP026656:85906-86169 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT42568 NZ_AP026656:85906-86169 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A133CER3

Antitoxin

• AlphaFold DB: A0A133CED7