Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 64262..64797 Replicon plasmid pAA94_1
Accession NZ_AP026613
Organism Enterococcus faecium strain AA94

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag OQH77_RS14555 Protein ID WP_002314366.1
Coordinates 64262..64534 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag OQH77_RS14560 Protein ID WP_002297422.1
Coordinates 64534..64797 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OQH77_RS14515 (EfmAA94_28550) 59512..59991 - 480 Protein_62 type IV secretory system conjugative DNA transfer family protein -
OQH77_RS14520 (EfmAA94_28560) 59988..60464 - 477 WP_002317294.1 PcfB family protein -
OQH77_RS14525 (EfmAA94_28570) 60539..60751 - 213 WP_002317295.1 DUF5348 domain-containing protein -
OQH77_RS14530 (EfmAA94_28580) 60773..61973 - 1201 Protein_65 helix-turn-helix domain-containing protein -
OQH77_RS14535 (EfmAA94_28600) 62250..62441 - 192 Protein_66 hypothetical protein -
OQH77_RS14540 (EfmAA94_28610) 62442..63299 - 858 WP_002292680.1 ParA family protein -
OQH77_RS14545 63713..63866 - 154 Protein_68 type II toxin-antitoxin system RelE/ParE family toxin -
OQH77_RS14550 (EfmAA94_28620) 63856..64185 - 330 WP_002292678.1 AbrB family transcriptional regulator -
OQH77_RS14555 (EfmAA94_28630) 64262..64534 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
OQH77_RS14560 (EfmAA94_28640) 64534..64797 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
OQH77_RS14565 65031..65938 + 908 Protein_72 IS982 family transposase -
OQH77_RS14570 (EfmAA94_28680) 65941..67116 - 1176 WP_002336205.1 MFS transporter -
OQH77_RS14575 (EfmAA94_28690) 67119..69368 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid VanHBX / ant(6)-Ia / erm(B) - 1..321364 321364


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T42561 WP_002314366.1 NZ_AP026613:c64534-64262 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T42561 NZ_AP026613:c64534-64262 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT42561 WP_002297422.1 NZ_AP026613:c64797-64534 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT42561 NZ_AP026613:c64797-64534 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References