Detailed information of TA system TA042550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 68504..69039
• Replicon: plasmid pJHP9_1
• Accession: NZ_AP026567
• Organism: Enterococcus faecium strain JHP9

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q3Y221
• Locus tag: OQH78_RS14890
• Protein ID: WP_002305108.1
• Coordinates: 68504..68776 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q3Y220
• Locus tag: OQH78_RS14895
• Protein ID: WP_002297422.1
• Coordinates: 68776..69039 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OQH78_RS14855 (EfmJHP9_29510)	64242..65312	+	1071	WP_002330546.1	sigma 54-interacting transcriptional regulator	-
OQH78_RS14860	65269..65364	-	96	Protein_62	S8 family serine peptidase	-
OQH78_RS14865 (EfmJHP9_29520)	65408..65869	-	462	WP_002298224.1	hypothetical protein	-
OQH78_RS14870 (EfmJHP9_29530)	66129..66677	-	549	WP_002292681.1	hypothetical protein	-
OQH78_RS14875 (EfmJHP9_29540)	66678..67535	-	858	WP_002292680.1	ParA family protein	-
OQH78_RS14880 (EfmJHP9_29550)	67821..68108	-	288	WP_002300557.1	type II toxin-antitoxin system RelE/ParE family toxin	-
OQH78_RS14885 (EfmJHP9_29560)	68098..68427	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
OQH78_RS14890 (EfmJHP9_29570)	68504..68776	-	273	WP_002305108.1	Txe/YoeB family addiction module toxin	Toxin
OQH78_RS14895 (EfmJHP9_29580)	68776..69039	-	264	WP_002297422.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
OQH78_RS14900 (EfmJHP9_29590)	69145..69468	-	324	WP_265168994.1	hypothetical protein	-
OQH78_RS14905 (EfmJHP9_29600)	69567..70130	-	564	Protein_71	IS6 family transposase	-
OQH78_RS14910	70210..71371	+	1162	Protein_72	IS3 family transposase	-
OQH78_RS14915 (EfmJHP9_29640)	71515..71952	-	438	WP_002307485.1	universal stress protein	-
OQH78_RS14920 (EfmJHP9_29650)	71961..73568	-	1608	WP_265168995.1	Nramp family divalent metal transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..258381	258381

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10806.28 Da
• Isoelectric Point: 9.9080

>T42550 WP_002305108.1 NZ_AP026567:c68776-68504 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T42550 NZ_AP026567:c68776-68504 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9959.22 Da
• Isoelectric Point: 4.5370

>AT42550 WP_002297422.1 NZ_AP026567:c69039-68776 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT42550 NZ_AP026567:c69039-68776 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A133CER3

Antitoxin

• AlphaFold DB: A0A829F006