Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3835207..3835804 Replicon chromosome
Accession NZ_AP026525
Organism Klebsiella pneumoniae subsp. pneumoniae strain TUM9839

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag OO859_RS18780 Protein ID WP_004142563.1
Coordinates 3835487..3835804 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag OO859_RS18775 Protein ID WP_004142561.1
Coordinates 3835207..3835494 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO859_RS18745 (TUM9839_36140) 3831287..3831535 + 249 WP_002893037.1 DUF1158 domain-containing protein -
OO859_RS18750 (TUM9839_36150) 3831553..3831894 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
OO859_RS18755 (TUM9839_36160) 3831925..3833040 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
OO859_RS18760 (TUM9839_36170) 3833220..3833801 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
OO859_RS18765 (TUM9839_36180) 3833801..3834169 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
OO859_RS18770 (TUM9839_36190) 3834289..3834942 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
OO859_RS18775 (TUM9839_36200) 3835207..3835494 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
OO859_RS18780 (TUM9839_36210) 3835487..3835804 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OO859_RS18785 (TUM9839_36220) 3835989..3837032 - 1044 WP_023284821.1 DUF2157 domain-containing protein -
OO859_RS18790 (TUM9839_36230) 3837698..3838564 - 867 WP_032436988.1 helix-turn-helix transcriptional regulator -
OO859_RS18795 (TUM9839_36240) 3838673..3840100 + 1428 WP_032411778.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T42531 WP_004142563.1 NZ_AP026525:c3835804-3835487 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T42531 NZ_AP026525:c3835804-3835487 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT42531 WP_004142561.1 NZ_AP026525:c3835494-3835207 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT42531 NZ_AP026525:c3835494-3835207 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTAGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References