Detailed information of TA system TA042472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3839253..3839850
• Replicon: chromosome
• Accession: NZ_AP026392
• Organism: Klebsiella quasipneumoniae subsp. quasipneumoniae strain KAM622

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: P4I30_RS19355
• Protein ID: WP_049013659.1
• Coordinates: 3839533..3839850 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: P4I30_RS19350
• Protein ID: WP_049013658.1
• Coordinates: 3839253..3839540 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P4I30_RS19315 (3835034)	3835034..3835342	-	309	Protein_3797	cation-transporting P-type ATPase	-
P4I30_RS19320 (3835441)	3835441..3835689	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
P4I30_RS19325 (3835707)	3835707..3836048	-	342	WP_004202492.1	RamA family antibiotic efflux transcriptional regulator	-
P4I30_RS19330 (3836079)	3836079..3837194	-	1116	WP_181075128.1	MBL fold metallo-hydrolase	-
P4I30_RS19335 (3837374)	3837374..3837958	+	585	WP_023288732.1	TetR/AcrR family transcriptional regulator	-
P4I30_RS19340 (3837955)	3837955..3838323	+	369	WP_004202487.1	MmcQ/YjbR family DNA-binding protein	-
P4I30_RS19345 (3838443)	3838443..3839096	+	654	WP_023288731.1	oxygen-insensitive NAD(P)H nitroreductase	-
P4I30_RS19350 (3839253)	3839253..3839540	-	288	WP_049013658.1	helix-turn-helix transcriptional regulator	Antitoxin
P4I30_RS19355 (3839533)	3839533..3839850	-	318	WP_049013659.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
P4I30_RS19360 (3840035)	3840035..3841078	-	1044	WP_261901673.1	DUF2157 domain-containing protein	-
P4I30_RS19365 (3841423)	3841423..3842289	-	867	WP_023288725.1	helix-turn-helix transcriptional regulator	-
P4I30_RS19370 (3842398)	3842398..3843825	+	1428	WP_048296961.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12102.38 Da
• Isoelectric Point: 11.2767

>T42472 WP_049013659.1 NZ_AP026392:c3839850-3839533 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MFRMVVHVDVKKELQALPAIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T42472 NZ_AP026392:c3839850-3839533 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGTTCAGAATGGTTGTGCACGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGGCCATTGTCCAGGCGAAGATGATCAG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGATAGTAAACCGCTGGGGCAGGGGCTATTCGAAA
TTCGGGCGTTAGGTTCGGTACAGGGGCGGGGGATTTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10810.49 Da
• Isoelectric Point: 10.2213

>AT42472 WP_049013658.1 NZ_AP026392:c3839540-3839253 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQHRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT42472 NZ_AP026392:c3839540-3839253 [Klebsiella quasipneumoniae subsp. quasipneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACGGAACTGCTCAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACATCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTATTAA