Detailed information of TA system TA042443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3953734..3954533
• Replicon: chromosome
• Accession: NZ_AP026118
• Organism: Escherichia coli strain CEC13004

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO879_RS19885
• Protein ID: WP_000347273.1
• Coordinates: 3954069..3954533 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO879_RS19880
• Protein ID: WP_001302819.1
• Coordinates: 3953734..3954069 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO879_RS19865 (3949519)	3949519..3950289	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO879_RS19870 (3950305)	3950305..3951639	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO879_RS19875 (3952014)	3952014..3953585	+	1572	WP_001273776.1	galactarate dehydratase	-
OO879_RS19880 (3953734)	3953734..3954069	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO879_RS19885 (3954069)	3954069..3954533	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO879_RS19890 (3954588)	3954588..3955397	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO879_RS19895 (3955646)	3955646..3956926	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO879_RS19900 (3956949)	3956949..3957422	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO879_RS19905 (3957433)	3957433..3958212	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO879_RS19910 (3958202)	3958202..3959080	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO879_RS19915 (3959098)	3959098..3959532	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T42443 WP_000347273.1 NZ_AP026118:3954069-3954533 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T42443 NZ_AP026118:3954069-3954533 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT42443 WP_001302819.1 NZ_AP026118:3953734-3954069 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT42443 NZ_AP026118:3953734-3954069 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7