Detailed information of TA system TA042419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 292364..293058
• Replicon: chromosome
• Accession: NZ_AP026118
• Organism: Escherichia coli strain CEC13004

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: OO879_RS01325
• Protein ID: WP_001263495.1
• Coordinates: 292660..293058 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: OO879_RS01320
• Protein ID: WP_000554757.1
• Coordinates: 292364..292657 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO879_RS01300 (288075)	288075..288572	+	498	Protein_252	REP-associated tyrosine transposase RayT	-
OO879_RS01305 (288717)	288717..290429	-	1713	Protein_253	flagellar biosynthesis protein FlhA	-
OO879_RS01310 (290401)	290401..291186	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
OO879_RS01315 (291257)	291257..292312	+	1056	WP_001226186.1	DNA polymerase IV	-
OO879_RS01320 (292364)	292364..292657	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
OO879_RS01325 (292660)	292660..293058	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
OO879_RS01330 (293068)	293068..293520	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
OO879_RS01335 (293839)	293839..294042	+	204	Protein_259	RtcB family protein	-
OO879_RS01340 (294041)	294041..294562	+	522	Protein_260	peptide chain release factor H	-
OO879_RS01345 (294619)	294619..296076	-	1458	WP_001293020.1	cytosol nonspecific dipeptidase	-
OO879_RS01350 (296337)	296337..296795	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (297391)	297391..297471	+	81	NuclAT_11	-	-
- (297391)	297391..297471	+	81	NuclAT_11	-	-
- (297391)	297391..297471	+	81	NuclAT_11	-	-
- (297391)	297391..297471	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T42419 WP_001263495.1 NZ_AP026118:292660-293058 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T42419 NZ_AP026118:292660-293058 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT42419 WP_000554757.1 NZ_AP026118:292364-292657 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT42419 NZ_AP026118:292364-292657 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6