Detailed information of TA system TA042369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3878570..3879369
• Replicon: chromosome
• Accession: NZ_AP026114
• Organism: Escherichia coli strain CEC01302

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO829_RS19335
• Protein ID: WP_000347273.1
• Coordinates: 3878905..3879369 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO829_RS19330
• Protein ID: WP_001302819.1
• Coordinates: 3878570..3878905 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO829_RS19315 (3874355)	3874355..3875125	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO829_RS19320 (3875141)	3875141..3876475	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO829_RS19325 (3876850)	3876850..3878421	+	1572	WP_001273776.1	galactarate dehydratase	-
OO829_RS19330 (3878570)	3878570..3878905	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO829_RS19335 (3878905)	3878905..3879369	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO829_RS19340 (3879424)	3879424..3880233	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO829_RS19345 (3880482)	3880482..3881762	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO829_RS19350 (3881785)	3881785..3882258	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO829_RS19355 (3882269)	3882269..3883048	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO829_RS19360 (3883038)	3883038..3883916	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO829_RS19365 (3883934)	3883934..3884368	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T42369 WP_000347273.1 NZ_AP026114:3878905-3879369 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T42369 NZ_AP026114:3878905-3879369 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT42369 WP_001302819.1 NZ_AP026114:3878570-3878905 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT42369 NZ_AP026114:3878570-3878905 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7