Detailed information of TA system TA042351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 292503..293197
• Replicon: chromosome
• Accession: NZ_AP026114
• Organism: Escherichia coli strain CEC01302

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: OO829_RS01325
• Protein ID: WP_001263495.1
• Coordinates: 292799..293197 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: OO829_RS01320
• Protein ID: WP_000554757.1
• Coordinates: 292503..292796 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO829_RS01300 (288214)	288214..288711	+	498	Protein_252	REP-associated tyrosine transposase RayT	-
OO829_RS01305 (288856)	288856..290568	-	1713	Protein_253	flagellar biosynthesis protein FlhA	-
OO829_RS01310 (290540)	290540..291325	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
OO829_RS01315 (291396)	291396..292451	+	1056	WP_001226186.1	DNA polymerase IV	-
OO829_RS01320 (292503)	292503..292796	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
OO829_RS01325 (292799)	292799..293197	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
OO829_RS01330 (293207)	293207..293659	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
OO829_RS01335 (293978)	293978..294181	+	204	Protein_259	RtcB family protein	-
OO829_RS01340 (294180)	294180..294701	+	522	Protein_260	peptide chain release factor H	-
OO829_RS01345 (294758)	294758..296215	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
OO829_RS01350 (296476)	296476..296934	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (297530)	297530..297610	+	81	NuclAT_11	-	-
- (297530)	297530..297610	+	81	NuclAT_11	-	-
- (297530)	297530..297610	+	81	NuclAT_11	-	-
- (297530)	297530..297610	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T42351 WP_001263495.1 NZ_AP026114:292799-293197 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T42351 NZ_AP026114:292799-293197 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT42351 WP_000554757.1 NZ_AP026114:292503-292796 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT42351 NZ_AP026114:292503-292796 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6