42342

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5190209..5190706 Replicon chromosome
Accession NZ_AP026112
Organism Escherichia coli strain CEC03102

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OO877_RS25980 Protein ID WP_001173343.1
Coordinates 5190533..5190706 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OO877_RS25975 Protein ID WP_000398619.1
Coordinates 5190209..5190505 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO877_RS25955 (5186940) 5186940..5187659 - 720 WP_000611288.1 two-component system response regulator DcuR -
OO877_RS25960 (5187656) 5187656..5189287 - 1632 WP_001216477.1 sensor histidine kinase -
OO877_RS25965 (5189468) 5189468..5189698 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
OO877_RS25970 (5189710) 5189710..5189982 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
OO877_RS25975 (5190209) 5190209..5190505 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OO877_RS25980 (5190533) 5190533..5190706 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OO877_RS25985 (5190825) 5190825..5192342 - 1518 WP_001295074.1 lysine--tRNA ligase -
OO877_RS25990 (5192579) 5192579..5194036 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 5190209..5199560 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T42342 WP_001173343.1 NZ_AP026112:5190533-5190706 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T42342 NZ_AP026112:5190533-5190706 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT42342 WP_000398619.1 NZ_AP026112:5190209-5190505 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT42342 NZ_AP026112:5190209-5190505 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References