Detailed information of TA system TA042336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4027353..4028152
• Replicon: chromosome
• Accession: NZ_AP026112
• Organism: Escherichia coli strain CEC03102

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO877_RS20360
• Protein ID: WP_000347273.1
• Coordinates: 4027688..4028152 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO877_RS20355
• Protein ID: WP_001302819.1
• Coordinates: 4027353..4027688 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO877_RS20340 (4023138)	4023138..4023908	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO877_RS20345 (4023924)	4023924..4025258	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO877_RS20350 (4025633)	4025633..4027204	+	1572	WP_001273776.1	galactarate dehydratase	-
OO877_RS20355 (4027353)	4027353..4027688	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO877_RS20360 (4027688)	4027688..4028152	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO877_RS20365 (4028207)	4028207..4029016	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO877_RS20370 (4029265)	4029265..4030545	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO877_RS20375 (4030568)	4030568..4031041	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO877_RS20380 (4031052)	4031052..4031831	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO877_RS20385 (4031821)	4031821..4032699	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO877_RS20390 (4032717)	4032717..4033151	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T42336 WP_000347273.1 NZ_AP026112:4027688-4028152 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T42336 NZ_AP026112:4027688-4028152 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT42336 WP_001302819.1 NZ_AP026112:4027353-4027688 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT42336 NZ_AP026112:4027353-4027688 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7