Detailed information of TA system TA042322

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2074543..2074768
• Replicon: chromosome
• Accession: NZ_AP026112
• Organism: Escherichia coli strain CEC03102

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: OO877_RS10210
• Protein ID: WP_000813258.1
• Coordinates: 2074543..2074698 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2074710..2074768 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO877_RS10160	2069546..2069977	-	432	WP_001302123.1	tellurite resistance protein	-
OO877_RS10175	2070428..2071141	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
OO877_RS10180	2071277..2071474	-	198	WP_000917763.1	hypothetical protein	-
OO877_RS10185	2071699..2072253	-	555	WP_000640035.1	DUF1133 family protein	-
OO877_RS10190	2072316..2072621	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
OO877_RS10195	2072634..2073683	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
OO877_RS10200	2073685..2073957	-	273	WP_000191871.1	hypothetical protein	-
OO877_RS10205	2074079..2074423	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
OO877_RS10210	2074543..2074698	-	156	WP_000813258.1	Hok/Gef family protein	Toxin
-	2074710..2074768	+	59	-	-	Antitoxin
OO877_RS10215	2074989..2075546	-	558	WP_000104474.1	DUF551 domain-containing protein	-
OO877_RS10220	2075548..2075766	-	219	WP_000683609.1	DUF4014 family protein	-
OO877_RS10225	2075894..2076205	-	312	WP_001289673.1	hypothetical protein	-
OO877_RS10230	2076198..2076425	-	228	WP_000699809.1	hypothetical protein	-
OO877_RS10235	2076422..2076703	-	282	WP_000603384.1	DNA-binding protein	-
OO877_RS10240	2076736..2077452	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
OO877_RS10245	2077486..2077947	-	462	WP_000139447.1	replication protein P	-
OO877_RS10250	2077940..2078995	-	1056	WP_001356791.1	DnaT-like ssDNA-binding domain-containing protein	-
OO877_RS10255	2079064..2079489	-	426	WP_000693878.1	toxin YdaT family protein	-
OO877_RS10260	2079473..2079716	-	244	Protein_2015	Cro/CI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2037097..2134133	97036

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T42322 WP_000813258.1 NZ_AP026112:c2074698-2074543 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T42322 NZ_AP026112:c2074698-2074543 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT42322 NZ_AP026112:2074710-2074768 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin