Detailed information of TA system TA042275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 292511..293205
• Replicon: chromosome
• Accession: NZ_AP026110
• Organism: Escherichia coli strain CEC08123

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: OO855_RS01325
• Protein ID: WP_001263495.1
• Coordinates: 292807..293205 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: OO855_RS01320
• Protein ID: WP_000554757.1
• Coordinates: 292511..292804 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO855_RS01300 (288222)	288222..288719	+	498	Protein_252	REP-associated tyrosine transposase RayT	-
OO855_RS01305 (288864)	288864..290576	-	1713	Protein_253	flagellar biosynthesis protein FlhA	-
OO855_RS01310 (290548)	290548..291333	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
OO855_RS01315 (291404)	291404..292459	+	1056	WP_001226186.1	DNA polymerase IV	-
OO855_RS01320 (292511)	292511..292804	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
OO855_RS01325 (292807)	292807..293205	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
OO855_RS01330 (293215)	293215..293667	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
OO855_RS01335 (293986)	293986..294189	+	204	Protein_259	RtcB family protein	-
OO855_RS01340 (294188)	294188..294709	+	522	Protein_260	peptide chain release factor H	-
OO855_RS01345 (294766)	294766..296223	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
OO855_RS01350 (296484)	296484..296942	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (297538)	297538..297618	+	81	NuclAT_11	-	-
- (297538)	297538..297618	+	81	NuclAT_11	-	-
- (297538)	297538..297618	+	81	NuclAT_11	-	-
- (297538)	297538..297618	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T42275 WP_001263495.1 NZ_AP026110:292807-293205 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T42275 NZ_AP026110:292807-293205 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT42275 WP_000554757.1 NZ_AP026110:292511-292804 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT42275 NZ_AP026110:292511-292804 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6