Detailed information of TA system TA042260

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4079000..4079799
• Replicon: chromosome
• Accession: NZ_AP026108
• Organism: Escherichia coli strain CEC13091

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO842_RS20600
• Protein ID: WP_000347273.1
• Coordinates: 4079335..4079799 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO842_RS20595
• Protein ID: WP_001302819.1
• Coordinates: 4079000..4079335 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO842_RS20580 (4074785)	4074785..4075555	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO842_RS20585 (4075571)	4075571..4076905	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO842_RS20590 (4077280)	4077280..4078851	+	1572	WP_055154484.1	galactarate dehydratase	-
OO842_RS20595 (4079000)	4079000..4079335	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO842_RS20600 (4079335)	4079335..4079799	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO842_RS20605 (4079854)	4079854..4080663	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO842_RS20610 (4080912)	4080912..4082192	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO842_RS20615 (4082215)	4082215..4082688	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO842_RS20620 (4082699)	4082699..4083478	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO842_RS20625 (4083468)	4083468..4084346	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO842_RS20630 (4084364)	4084364..4084798	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T42260 WP_000347273.1 NZ_AP026108:4079335-4079799 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T42260 NZ_AP026108:4079335-4079799 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT42260 WP_001302819.1 NZ_AP026108:4079000-4079335 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT42260 NZ_AP026108:4079000-4079335 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7