Detailed information of TA system TA042246

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2162376..2162601
• Replicon: chromosome
• Accession: NZ_AP026108
• Organism: Escherichia coli strain CEC13091

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: OO842_RS10720
• Protein ID: WP_000813258.1
• Coordinates: 2162376..2162531 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2162543..2162601 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO842_RS10670	2157379..2157810	-	432	WP_001302123.1	tellurite resistance protein	-
OO842_RS10685	2158261..2158974	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
OO842_RS10690	2159110..2159307	-	198	WP_000917763.1	hypothetical protein	-
OO842_RS10695	2159532..2160086	-	555	WP_000640035.1	DUF1133 family protein	-
OO842_RS10700	2160149..2160454	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
OO842_RS10705	2160467..2161516	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
OO842_RS10710	2161518..2161790	-	273	WP_000191871.1	hypothetical protein	-
OO842_RS10715	2161912..2162256	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
OO842_RS10720	2162376..2162531	-	156	WP_000813258.1	Hok/Gef family protein	Toxin
-	2162543..2162601	+	59	-	-	Antitoxin
OO842_RS10725	2162822..2163379	-	558	WP_000104474.1	DUF551 domain-containing protein	-
OO842_RS10730	2163381..2163599	-	219	WP_000683609.1	DUF4014 family protein	-
OO842_RS10735	2163727..2164038	-	312	WP_001289673.1	hypothetical protein	-
OO842_RS10740	2164031..2164258	-	228	WP_000699809.1	hypothetical protein	-
OO842_RS10745	2164255..2164536	-	282	WP_000603384.1	DNA-binding protein	-
OO842_RS10750	2164569..2165285	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
OO842_RS10755	2165319..2165780	-	462	WP_000139447.1	replication protein P	-
OO842_RS10760	2165773..2166828	-	1056	WP_001356791.1	DnaT-like ssDNA-binding domain-containing protein	-
OO842_RS10765	2166897..2167322	-	426	WP_000693878.1	toxin YdaT family protein	-
OO842_RS10770	2167306..2167549	-	244	Protein_2117	Cro/CI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2124929..2223088	98159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T42246 WP_000813258.1 NZ_AP026108:c2162531-2162376 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T42246 NZ_AP026108:c2162531-2162376 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT42246 NZ_AP026108:2162543-2162601 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin