Detailed information of TA system TA042182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3939712..3940511
• Replicon: chromosome
• Accession: NZ_AP026104
• Organism: Escherichia coli strain CEC96047

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO843_RS19830
• Protein ID: WP_000347273.1
• Coordinates: 3940047..3940511 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO843_RS19825
• Protein ID: WP_001302819.1
• Coordinates: 3939712..3940047 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO843_RS19810 (3935497)	3935497..3936267	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO843_RS19815 (3936283)	3936283..3937617	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO843_RS19820 (3937992)	3937992..3939563	+	1572	WP_001273776.1	galactarate dehydratase	-
OO843_RS19825 (3939712)	3939712..3940047	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO843_RS19830 (3940047)	3940047..3940511	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO843_RS19835 (3940566)	3940566..3941375	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO843_RS19840 (3941624)	3941624..3942904	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO843_RS19845 (3942927)	3942927..3943400	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO843_RS19850 (3943411)	3943411..3944190	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO843_RS19855 (3944180)	3944180..3945058	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO843_RS19860 (3945076)	3945076..3945510	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T42182 WP_000347273.1 NZ_AP026104:3940047-3940511 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T42182 NZ_AP026104:3940047-3940511 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT42182 WP_001302819.1 NZ_AP026104:3939712-3940047 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT42182 NZ_AP026104:3939712-3940047 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7