Detailed information of TA system TA042148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4061192..4061991
• Replicon: chromosome
• Accession: NZ_AP026101
• Organism: Escherichia coli strain 93_161312

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO868_RS20645
• Protein ID: WP_000347273.1
• Coordinates: 4061527..4061991 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO868_RS20640
• Protein ID: WP_001302819.1
• Coordinates: 4061192..4061527 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO868_RS20625 (4056977)	4056977..4057747	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO868_RS20630 (4057763)	4057763..4059097	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO868_RS20635 (4059472)	4059472..4061043	+	1572	WP_001273776.1	galactarate dehydratase	-
OO868_RS20640 (4061192)	4061192..4061527	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO868_RS20645 (4061527)	4061527..4061991	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO868_RS20650 (4062046)	4062046..4062855	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO868_RS20655 (4063104)	4063104..4064384	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO868_RS20660 (4064407)	4064407..4064880	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO868_RS20665 (4064891)	4064891..4065670	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO868_RS20670 (4065660)	4065660..4066538	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO868_RS20675 (4066556)	4066556..4066990	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T42148 WP_000347273.1 NZ_AP026101:4061527-4061991 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T42148 NZ_AP026101:4061527-4061991 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT42148 WP_001302819.1 NZ_AP026101:4061192-4061527 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT42148 NZ_AP026101:4061192-4061527 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7