Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4018314..4019041 Replicon chromosome
Accession NZ_AP026101
Organism Escherichia coli strain 93_161312

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag OO868_RS20420 Protein ID WP_000550189.1
Coordinates 4018727..4019041 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag OO868_RS20415 Protein ID WP_000560249.1
Coordinates 4018314..4018730 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO868_RS20405 (4013374) 4013374..4015725 + 2352 WP_000695517.1 alpha-glucosidase -
OO868_RS20410 (4016251) 4016251..4018269 + 2019 WP_000121479.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
OO868_RS20415 (4018314) 4018314..4018730 - 417 WP_000560249.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
OO868_RS20420 (4018727) 4018727..4019041 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
OO868_RS20425 (4019325) 4019325..4020461 - 1137 WP_000018678.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
OO868_RS20430 (4020546) 4020546..4021049 + 504 WP_001295542.1 M48 family metallopeptidase -
OO868_RS20435 (4021126) 4021126..4021818 + 693 WP_000942543.1 vancomycin high temperature exclusion protein -
OO868_RS20440 (4021897) 4021897..4022883 + 987 WP_001453073.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T42147 WP_000550189.1 NZ_AP026101:c4019041-4018727 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T42147 NZ_AP026101:c4019041-4018727 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15016.48 Da        Isoelectric Point: 4.5805

>AT42147 WP_000560249.1 NZ_AP026101:c4018730-4018314 [Escherichia coli]
MIAIADILHAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT42147 NZ_AP026101:c4018730-4018314 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References