Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 292365..293059 Replicon chromosome
Accession NZ_AP026101
Organism Escherichia coli strain 93_161312

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag OO868_RS01325 Protein ID WP_001263495.1
Coordinates 292661..293059 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag OO868_RS01320 Protein ID WP_000554757.1
Coordinates 292365..292658 (+) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO868_RS01300 (288076) 288076..288573 + 498 Protein_252 REP-associated tyrosine transposase RayT -
OO868_RS01305 (288718) 288718..290430 - 1713 Protein_253 flagellar biosynthesis protein FlhA -
OO868_RS01310 (290402) 290402..291187 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
OO868_RS01315 (291258) 291258..292313 + 1056 WP_001226186.1 DNA polymerase IV -
OO868_RS01320 (292365) 292365..292658 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
OO868_RS01325 (292661) 292661..293059 + 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
OO868_RS01330 (293069) 293069..293521 + 453 WP_001059871.1 GNAT family N-acetyltransferase -
OO868_RS01335 (293840) 293840..294043 + 204 Protein_259 RtcB family protein -
OO868_RS01340 (294042) 294042..294563 + 522 Protein_260 peptide chain release factor H -
OO868_RS01345 (294620) 294620..296077 - 1458 WP_001293020.1 cytosol nonspecific dipeptidase -
OO868_RS01350 (296338) 296338..296796 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (297392) 297392..297472 + 81 NuclAT_11 - -
- (297392) 297392..297472 + 81 NuclAT_11 - -
- (297392) 297392..297472 + 81 NuclAT_11 - -
- (297392) 297392..297472 + 81 NuclAT_11 - -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T42126 WP_001263495.1 NZ_AP026101:292661-293059 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T42126 NZ_AP026101:292661-293059 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT42126 WP_000554757.1 NZ_AP026101:292365-292658 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT42126 NZ_AP026101:292365-292658 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References