Detailed information of TA system TA042115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4170644..4171443
• Replicon: chromosome
• Accession: NZ_AP026099
• Organism: Escherichia coli strain 61_150228

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO862_RS21390
• Protein ID: WP_000347273.1
• Coordinates: 4170979..4171443 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO862_RS21385
• Protein ID: WP_001302819.1
• Coordinates: 4170644..4170979 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO862_RS21370 (4166429)	4166429..4167199	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO862_RS21375 (4167215)	4167215..4168549	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO862_RS21380 (4168924)	4168924..4170495	+	1572	WP_001273776.1	galactarate dehydratase	-
OO862_RS21385 (4170644)	4170644..4170979	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO862_RS21390 (4170979)	4170979..4171443	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO862_RS21395 (4171498)	4171498..4172307	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO862_RS21400 (4172556)	4172556..4173836	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO862_RS21405 (4173859)	4173859..4174332	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO862_RS21410 (4174343)	4174343..4175122	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO862_RS21415 (4175112)	4175112..4175990	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO862_RS21420 (4176008)	4176008..4176442	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T42115 WP_000347273.1 NZ_AP026099:4170979-4171443 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T42115 NZ_AP026099:4170979-4171443 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT42115 WP_001302819.1 NZ_AP026099:4170644-4170979 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT42115 NZ_AP026099:4170644-4170979 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7