Detailed information of TA system TA042031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 687206..688005
• Replicon: chromosome
• Accession: NZ_AP026095
• Organism: Escherichia coli strain 53_142304

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO816_RS03240
• Protein ID: WP_000347273.1
• Coordinates: 687206..687670 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO816_RS03245
• Protein ID: WP_001302819.1
• Coordinates: 687670..688005 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO816_RS03210 (682207)	682207..682641	-	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
OO816_RS03215 (682659)	682659..683537	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO816_RS03220 (683527)	683527..684306	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO816_RS03225 (684317)	684317..684790	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO816_RS03230 (684813)	684813..686093	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO816_RS03235 (686342)	686342..687151	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO816_RS03240 (687206)	687206..687670	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO816_RS03245 (687670)	687670..688005	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO816_RS03250 (688154)	688154..689725	-	1572	WP_001273776.1	galactarate dehydratase	-
OO816_RS03255 (690100)	690100..691434	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO816_RS03260 (691450)	691450..692220	+	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T42031 WP_000347273.1 NZ_AP026095:c687670-687206 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T42031 NZ_AP026095:c687670-687206 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT42031 WP_001302819.1 NZ_AP026095:c688005-687670 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT42031 NZ_AP026095:c688005-687670 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7