Detailed information of TA system TA042010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3956884..3957683
• Replicon: chromosome
• Accession: NZ_AP026092
• Organism: Escherichia coli strain 27_141091

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO959_RS19875
• Protein ID: WP_000347273.1
• Coordinates: 3957219..3957683 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO959_RS19870
• Protein ID: WP_001302819.1
• Coordinates: 3956884..3957219 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO959_RS19855 (3952669)	3952669..3953439	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO959_RS19860 (3953455)	3953455..3954789	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO959_RS19865 (3955164)	3955164..3956735	+	1572	WP_001273776.1	galactarate dehydratase	-
OO959_RS19870 (3956884)	3956884..3957219	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO959_RS19875 (3957219)	3957219..3957683	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO959_RS19880 (3957738)	3957738..3958547	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO959_RS19885 (3958796)	3958796..3960076	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO959_RS19890 (3960099)	3960099..3960572	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO959_RS19895 (3960583)	3960583..3961362	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO959_RS19900 (3961352)	3961352..3962230	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO959_RS19905 (3962248)	3962248..3962682	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T42010 WP_000347273.1 NZ_AP026092:3957219-3957683 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T42010 NZ_AP026092:3957219-3957683 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT42010 WP_001302819.1 NZ_AP026092:3956884-3957219 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT42010 NZ_AP026092:3956884-3957219 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7