Detailed information of TA system TA041988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 292530..293224
• Replicon: chromosome
• Accession: NZ_AP026092
• Organism: Escherichia coli strain 27_141091

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: OO959_RS01325
• Protein ID: WP_001263495.1
• Coordinates: 292826..293224 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: OO959_RS01320
• Protein ID: WP_000554757.1
• Coordinates: 292530..292823 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO959_RS01300 (288241)	288241..288738	+	498	Protein_252	REP-associated tyrosine transposase RayT	-
OO959_RS01305 (288883)	288883..290595	-	1713	Protein_253	flagellar biosynthesis protein FlhA	-
OO959_RS01310 (290567)	290567..291352	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
OO959_RS01315 (291423)	291423..292478	+	1056	WP_001226186.1	DNA polymerase IV	-
OO959_RS01320 (292530)	292530..292823	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
OO959_RS01325 (292826)	292826..293224	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
OO959_RS01330 (293234)	293234..293686	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
OO959_RS01335 (294005)	294005..294208	+	204	Protein_259	RtcB family protein	-
OO959_RS01340 (294207)	294207..294728	+	522	Protein_260	peptide chain release factor H	-
OO959_RS01345 (294785)	294785..296242	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
OO959_RS01350 (296503)	296503..296961	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (297557)	297557..297637	+	81	NuclAT_12	-	-
- (297557)	297557..297637	+	81	NuclAT_12	-	-
- (297557)	297557..297637	+	81	NuclAT_12	-	-
- (297557)	297557..297637	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T41988 WP_001263495.1 NZ_AP026092:292826-293224 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T41988 NZ_AP026092:292826-293224 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT41988 WP_000554757.1 NZ_AP026092:292530-292823 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT41988 NZ_AP026092:292530-292823 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6