Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5187435..5187932 Replicon chromosome
Accession NZ_AP026087
Organism Escherichia coli strain 8_140198

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag OO841_RS26125 Protein ID WP_001173343.1
Coordinates 5187759..5187932 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag OO841_RS26120 Protein ID WP_000398619.1
Coordinates 5187435..5187731 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OO841_RS26100 (5184166) 5184166..5184885 - 720 WP_000611288.1 two-component system response regulator DcuR -
OO841_RS26105 (5184882) 5184882..5186513 - 1632 WP_001216477.1 sensor histidine kinase -
OO841_RS26110 (5186694) 5186694..5186924 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
OO841_RS26115 (5186936) 5186936..5187208 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
OO841_RS26120 (5187435) 5187435..5187731 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
OO841_RS26125 (5187759) 5187759..5187932 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
OO841_RS26130 (5188051) 5188051..5189568 - 1518 WP_001295074.1 lysine--tRNA ligase -
OO841_RS26135 (5189805) 5189805..5191262 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 5187435..5196786 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T41941 WP_001173343.1 NZ_AP026087:5187759-5187932 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T41941 NZ_AP026087:5187759-5187932 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT41941 WP_000398619.1 NZ_AP026087:5187435-5187731 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT41941 NZ_AP026087:5187435-5187731 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References