Detailed information of TA system TA041935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4024501..4025300
• Replicon: chromosome
• Accession: NZ_AP026087
• Organism: Escherichia coli strain 8_140198

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO841_RS20505
• Protein ID: WP_000347273.1
• Coordinates: 4024836..4025300 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO841_RS20500
• Protein ID: WP_001302819.1
• Coordinates: 4024501..4024836 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO841_RS20485 (4020286)	4020286..4021056	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO841_RS20490 (4021072)	4021072..4022406	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO841_RS20495 (4022781)	4022781..4024352	+	1572	WP_001273776.1	galactarate dehydratase	-
OO841_RS20500 (4024501)	4024501..4024836	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO841_RS20505 (4024836)	4024836..4025300	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO841_RS20510 (4025355)	4025355..4026164	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO841_RS20515 (4026413)	4026413..4027693	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO841_RS20520 (4027716)	4027716..4028189	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO841_RS20525 (4028200)	4028200..4028979	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO841_RS20530 (4028969)	4028969..4029847	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO841_RS20535 (4029865)	4029865..4030299	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T41935 WP_000347273.1 NZ_AP026087:4024836-4025300 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T41935 NZ_AP026087:4024836-4025300 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT41935 WP_001302819.1 NZ_AP026087:4024501-4024836 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT41935 NZ_AP026087:4024501-4024836 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7