Detailed information of TA system TA041894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3959986..3960785
• Replicon: chromosome
• Accession: NZ_AP026085
• Organism: Escherichia coli strain H52_982342

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO840_RS19915
• Protein ID: WP_000347273.1
• Coordinates: 3960321..3960785 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO840_RS19910
• Protein ID: WP_001302819.1
• Coordinates: 3959986..3960321 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO840_RS19895 (3955771)	3955771..3956541	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO840_RS19900 (3956557)	3956557..3957891	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO840_RS19905 (3958266)	3958266..3959837	+	1572	WP_001273776.1	galactarate dehydratase	-
OO840_RS19910 (3959986)	3959986..3960321	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO840_RS19915 (3960321)	3960321..3960785	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO840_RS19920 (3960840)	3960840..3961649	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO840_RS19925 (3961898)	3961898..3963178	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO840_RS19930 (3963201)	3963201..3963674	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO840_RS19935 (3963685)	3963685..3964464	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO840_RS19940 (3964454)	3964454..3965332	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO840_RS19945 (3965350)	3965350..3965784	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T41894 WP_000347273.1 NZ_AP026085:3960321-3960785 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T41894 NZ_AP026085:3960321-3960785 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT41894 WP_001302819.1 NZ_AP026085:3959986-3960321 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT41894 NZ_AP026085:3959986-3960321 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7