Detailed information of TA system TA041859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4003600..4004399
• Replicon: chromosome
• Accession: NZ_AP026082
• Organism: Escherichia coli strain F765

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO934_RS20240
• Protein ID: WP_000347273.1
• Coordinates: 4003935..4004399 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO934_RS20235
• Protein ID: WP_001302819.1
• Coordinates: 4003600..4003935 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO934_RS20220 (3999385)	3999385..4000155	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO934_RS20225 (4000171)	4000171..4001505	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO934_RS20230 (4001880)	4001880..4003451	+	1572	WP_001273776.1	galactarate dehydratase	-
OO934_RS20235 (4003600)	4003600..4003935	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO934_RS20240 (4003935)	4003935..4004399	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO934_RS20245 (4004454)	4004454..4005263	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO934_RS20250 (4005512)	4005512..4006792	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO934_RS20255 (4006815)	4006815..4007288	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO934_RS20260 (4007299)	4007299..4008078	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO934_RS20265 (4008068)	4008068..4008946	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO934_RS20270 (4008964)	4008964..4009398	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T41859 WP_000347273.1 NZ_AP026082:4003935-4004399 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T41859 NZ_AP026082:4003935-4004399 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT41859 WP_001302819.1 NZ_AP026082:4003600-4003935 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT41859 NZ_AP026082:4003600-4003935 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7