Detailed information of TA system TA041820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4002381..4003180
• Replicon: chromosome
• Accession: NZ_AP026080
• Organism: Escherichia coli strain F690

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: OO857_RS20175
• Protein ID: WP_000347273.1
• Coordinates: 4002716..4003180 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: OO857_RS20170
• Protein ID: WP_001302819.1
• Coordinates: 4002381..4002716 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OO857_RS20155 (3998166)	3998166..3998936	-	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-
OO857_RS20160 (3998952)	3998952..4000286	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
OO857_RS20165 (4000661)	4000661..4002232	+	1572	WP_001273776.1	galactarate dehydratase	-
OO857_RS20170 (4002381)	4002381..4002716	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
OO857_RS20175 (4002716)	4002716..4003180	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
OO857_RS20180 (4003235)	4003235..4004044	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
OO857_RS20185 (4004293)	4004293..4005573	+	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
OO857_RS20190 (4005596)	4005596..4006069	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
OO857_RS20195 (4006080)	4006080..4006859	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
OO857_RS20200 (4006849)	4006849..4007727	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
OO857_RS20205 (4007745)	4007745..4008179	+	435	WP_000948831.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T41820 WP_000347273.1 NZ_AP026080:4002716-4003180 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T41820 NZ_AP026080:4002716-4003180 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT41820 WP_001302819.1 NZ_AP026080:4002381-4002716 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT41820 NZ_AP026080:4002381-4002716 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7