Detailed information of TA system TA041345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4771600..4772202
• Replicon: chromosome
• Accession: NZ_AP025842
• Organism: Enterobacter hormaechei strain FUJ80379

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NMC33_RS23045
• Protein ID: WP_022649837.1
• Coordinates: 4771891..4772202 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NMC33_RS23040
• Protein ID: WP_022649836.1
• Coordinates: 4771600..4771890 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NMC33_RS23025 (FJMB80379_45130)	4769098..4770000	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NMC33_RS23030 (FJMB80379_45140)	4769997..4770632	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NMC33_RS23035 (FJMB80379_45150)	4770629..4771558	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NMC33_RS23040 (FJMB80379_45160)	4771600..4771890	-	291	WP_022649836.1	NadS family protein	Antitoxin
NMC33_RS23045 (FJMB80379_45170)	4771891..4772202	-	312	WP_022649837.1	hypothetical protein	Toxin
NMC33_RS23050 (FJMB80379_45180)	4772351..4773292	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NMC33_RS23055 (FJMB80379_45190)	4773337..4773774	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NMC33_RS23060 (FJMB80379_45200)	4773771..4774652	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NMC33_RS23065 (FJMB80379_45210)	4774646..4775245	-	600	WP_022649841.1	glucose-1-phosphatase	-
NMC33_RS23070 (FJMB80379_45220)	4775364..4776164	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NMC33_RS23075 (FJMB80379_45230)	4776199..4777095	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T41345 WP_022649837.1 NZ_AP025842:c4772202-4771891 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T41345 NZ_AP025842:c4772202-4771891 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT41345 WP_022649836.1 NZ_AP025842:c4771890-4771600 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT41345 NZ_AP025842:c4771890-4771600 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6