Detailed information of TA system TA041253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4730181..4730783
• Replicon: chromosome
• Accession: NZ_AP025824
• Organism: Enterobacter hormaechei strain FUJ80067

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGJ94_RS22775
• Protein ID: WP_022649837.1
• Coordinates: 4730472..4730783 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGJ94_RS22770
• Protein ID: WP_022649836.1
• Coordinates: 4730181..4730471 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGJ94_RS22755 (FJMB80067_44830)	4727679..4728581	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGJ94_RS22760 (FJMB80067_44840)	4728578..4729213	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGJ94_RS22765 (FJMB80067_44850)	4729210..4730139	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGJ94_RS22770 (FJMB80067_44860)	4730181..4730471	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGJ94_RS22775 (FJMB80067_44870)	4730472..4730783	-	312	WP_022649837.1	hypothetical protein	Toxin
NGJ94_RS22780 (FJMB80067_44880)	4730932..4731873	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGJ94_RS22785 (FJMB80067_44890)	4731918..4732355	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGJ94_RS22790 (FJMB80067_44900)	4732352..4733233	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGJ94_RS22795 (FJMB80067_44910)	4733227..4733826	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGJ94_RS22800 (FJMB80067_44920)	4733945..4734745	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGJ94_RS22805 (FJMB80067_44930)	4734780..4735676	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T41253 WP_022649837.1 NZ_AP025824:c4730783-4730472 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T41253 NZ_AP025824:c4730783-4730472 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT41253 WP_022649836.1 NZ_AP025824:c4730471-4730181 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT41253 NZ_AP025824:c4730471-4730181 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6