Detailed information of TA system TA041237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4666632..4667234
• Replicon: chromosome
• Accession: NZ_AP025821
• Organism: Enterobacter hormaechei strain FUJ80066

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGK28_RS22340
• Protein ID: WP_022649837.1
• Coordinates: 4666923..4667234 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGK28_RS22335
• Protein ID: WP_022649836.1
• Coordinates: 4666632..4666922 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK28_RS22320 (FJMB80066_43630)	4664130..4665032	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGK28_RS22325 (FJMB80066_43640)	4665029..4665664	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGK28_RS22330 (FJMB80066_43650)	4665661..4666590	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGK28_RS22335 (FJMB80066_43660)	4666632..4666922	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGK28_RS22340 (FJMB80066_43670)	4666923..4667234	-	312	WP_022649837.1	hypothetical protein	Toxin
NGK28_RS22345 (FJMB80066_43680)	4667383..4668324	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGK28_RS22350 (FJMB80066_43690)	4668369..4668806	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGK28_RS22355 (FJMB80066_43700)	4668803..4669684	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGK28_RS22360 (FJMB80066_43710)	4669678..4670277	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGK28_RS22365 (FJMB80066_43720)	4670396..4671196	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGK28_RS22370 (FJMB80066_43730)	4671231..4672127	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T41237 WP_022649837.1 NZ_AP025821:c4667234-4666923 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T41237 NZ_AP025821:c4667234-4666923 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT41237 WP_022649836.1 NZ_AP025821:c4666922-4666632 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT41237 NZ_AP025821:c4666922-4666632 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6