Detailed information of TA system TA041221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4632151..4632753
• Replicon: chromosome
• Accession: NZ_AP025818
• Organism: Enterobacter hormaechei strain FUJ80065

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGJ50_RS22035
• Protein ID: WP_022649837.1
• Coordinates: 4632442..4632753 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGJ50_RS22030
• Protein ID: WP_022649836.1
• Coordinates: 4632151..4632441 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGJ50_RS22015 (FJMB80065_42950)	4629649..4630551	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGJ50_RS22020 (FJMB80065_42960)	4630548..4631183	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGJ50_RS22025 (FJMB80065_42970)	4631180..4632109	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGJ50_RS22030 (FJMB80065_42980)	4632151..4632441	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGJ50_RS22035 (FJMB80065_42990)	4632442..4632753	-	312	WP_022649837.1	hypothetical protein	Toxin
NGJ50_RS22040 (FJMB80065_43000)	4632902..4633843	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGJ50_RS22045 (FJMB80065_43010)	4633888..4634325	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGJ50_RS22050 (FJMB80065_43020)	4634322..4635203	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGJ50_RS22055 (FJMB80065_43030)	4635197..4635796	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGJ50_RS22060 (FJMB80065_43040)	4635915..4636715	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGJ50_RS22065 (FJMB80065_43050)	4636750..4637646	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T41221 WP_022649837.1 NZ_AP025818:c4632753-4632442 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T41221 NZ_AP025818:c4632753-4632442 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT41221 WP_022649836.1 NZ_AP025818:c4632441-4632151 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT41221 NZ_AP025818:c4632441-4632151 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6