Detailed information of TA system TA041205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4641310..4641912
• Replicon: chromosome
• Accession: NZ_AP025815
• Organism: Enterobacter hormaechei strain FUJ80064

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGJ20_RS22085
• Protein ID: WP_022649837.1
• Coordinates: 4641601..4641912 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGJ20_RS22080
• Protein ID: WP_022649836.1
• Coordinates: 4641310..4641600 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGJ20_RS22065 (FJMB80064_43030)	4638808..4639710	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGJ20_RS22070 (FJMB80064_43040)	4639707..4640342	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGJ20_RS22075 (FJMB80064_43050)	4640339..4641268	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGJ20_RS22080 (FJMB80064_43060)	4641310..4641600	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGJ20_RS22085 (FJMB80064_43070)	4641601..4641912	-	312	WP_022649837.1	hypothetical protein	Toxin
NGJ20_RS22090 (FJMB80064_43080)	4642061..4643002	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGJ20_RS22095 (FJMB80064_43090)	4643047..4643484	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGJ20_RS22100 (FJMB80064_43100)	4643481..4644362	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGJ20_RS22105 (FJMB80064_43110)	4644356..4644955	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGJ20_RS22110 (FJMB80064_43120)	4645074..4645874	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGJ20_RS22115 (FJMB80064_43130)	4645909..4646805	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T41205 WP_022649837.1 NZ_AP025815:c4641912-4641601 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T41205 NZ_AP025815:c4641912-4641601 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT41205 WP_022649836.1 NZ_AP025815:c4641600-4641310 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT41205 NZ_AP025815:c4641600-4641310 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6