Detailed information of TA system TA041189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4645701..4646303
• Replicon: chromosome
• Accession: NZ_AP025812
• Organism: Enterobacter hormaechei strain FUJ80062

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGK32_RS22180
• Protein ID: WP_022649837.1
• Coordinates: 4645992..4646303 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGK32_RS22175
• Protein ID: WP_022649836.1
• Coordinates: 4645701..4645991 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK32_RS22160 (FJMB80062_43200)	4643199..4644101	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGK32_RS22165 (FJMB80062_43210)	4644098..4644733	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGK32_RS22170 (FJMB80062_43220)	4644730..4645659	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGK32_RS22175 (FJMB80062_43230)	4645701..4645991	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGK32_RS22180 (FJMB80062_43240)	4645992..4646303	-	312	WP_022649837.1	hypothetical protein	Toxin
NGK32_RS22185 (FJMB80062_43250)	4646452..4647393	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGK32_RS22190 (FJMB80062_43260)	4647438..4647875	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGK32_RS22195 (FJMB80062_43270)	4647872..4648753	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGK32_RS22200 (FJMB80062_43280)	4648747..4649346	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGK32_RS22205 (FJMB80062_43290)	4649465..4650265	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGK32_RS22210 (FJMB80062_43300)	4650300..4651196	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T41189 WP_022649837.1 NZ_AP025812:c4646303-4645992 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T41189 NZ_AP025812:c4646303-4645992 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT41189 WP_022649836.1 NZ_AP025812:c4645991-4645701 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT41189 NZ_AP025812:c4645991-4645701 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6