Detailed information of TA system TA041141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4571544..4572146
• Replicon: chromosome
• Accession: NZ_AP025804
• Organism: Enterobacter hormaechei strain FUJ80053

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGK45_RS21690
• Protein ID: WP_022649837.1
• Coordinates: 4571835..4572146 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGK45_RS21685
• Protein ID: WP_022649836.1
• Coordinates: 4571544..4571834 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK45_RS21670 (FJMB80053_42360)	4569042..4569944	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGK45_RS21675 (FJMB80053_42370)	4569941..4570576	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGK45_RS21680 (FJMB80053_42380)	4570573..4571502	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGK45_RS21685 (FJMB80053_42390)	4571544..4571834	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGK45_RS21690 (FJMB80053_42400)	4571835..4572146	-	312	WP_022649837.1	hypothetical protein	Toxin
NGK45_RS21695 (FJMB80053_42410)	4572295..4573236	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGK45_RS21700 (FJMB80053_42420)	4573281..4573718	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGK45_RS21705 (FJMB80053_42430)	4573715..4574596	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGK45_RS21710 (FJMB80053_42440)	4574590..4575189	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGK45_RS21715 (FJMB80053_42450)	4575308..4576108	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGK45_RS21720 (FJMB80053_42460)	4576143..4577039	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T41141 WP_022649837.1 NZ_AP025804:c4572146-4571835 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T41141 NZ_AP025804:c4572146-4571835 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT41141 WP_022649836.1 NZ_AP025804:c4571834-4571544 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT41141 NZ_AP025804:c4571834-4571544 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6