Detailed information of TA system TA041125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4641331..4641933
• Replicon: chromosome
• Accession: NZ_AP025801
• Organism: Enterobacter hormaechei strain FUJ80024

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGJ01_RS22085
• Protein ID: WP_022649837.1
• Coordinates: 4641622..4641933 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGJ01_RS22080
• Protein ID: WP_022649836.1
• Coordinates: 4641331..4641621 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGJ01_RS22065 (FJMB80024_43340)	4638829..4639731	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGJ01_RS22070 (FJMB80024_43350)	4639728..4640363	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGJ01_RS22075 (FJMB80024_43360)	4640360..4641289	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGJ01_RS22080 (FJMB80024_43370)	4641331..4641621	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGJ01_RS22085 (FJMB80024_43380)	4641622..4641933	-	312	WP_022649837.1	hypothetical protein	Toxin
NGJ01_RS22090 (FJMB80024_43390)	4642082..4643023	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGJ01_RS22095 (FJMB80024_43400)	4643068..4643505	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGJ01_RS22100 (FJMB80024_43410)	4643502..4644383	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGJ01_RS22105 (FJMB80024_43420)	4644377..4644976	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGJ01_RS22110 (FJMB80024_43430)	4645095..4645895	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGJ01_RS22115 (FJMB80024_43440)	4645930..4646826	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T41125 WP_022649837.1 NZ_AP025801:c4641933-4641622 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T41125 NZ_AP025801:c4641933-4641622 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT41125 WP_022649836.1 NZ_AP025801:c4641621-4641331 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT41125 NZ_AP025801:c4641621-4641331 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6