Detailed information of TA system TA041109

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4704610..4705212
• Replicon: chromosome
• Accession: NZ_AP025799
• Organism: Enterobacter hormaechei strain FUJ80023

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGJ00_RS22620
• Protein ID: WP_022649837.1
• Coordinates: 4704901..4705212 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGJ00_RS22615
• Protein ID: WP_022649836.1
• Coordinates: 4704610..4704900 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGJ00_RS22600 (FJMB80023_44160)	4702108..4703010	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGJ00_RS22605 (FJMB80023_44170)	4703007..4703642	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGJ00_RS22610 (FJMB80023_44180)	4703639..4704568	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGJ00_RS22615 (FJMB80023_44190)	4704610..4704900	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGJ00_RS22620 (FJMB80023_44200)	4704901..4705212	-	312	WP_022649837.1	hypothetical protein	Toxin
NGJ00_RS22625 (FJMB80023_44210)	4705361..4706302	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGJ00_RS22630 (FJMB80023_44220)	4706347..4706784	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGJ00_RS22635 (FJMB80023_44230)	4706781..4707662	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGJ00_RS22640 (FJMB80023_44240)	4707656..4708255	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGJ00_RS22645 (FJMB80023_44250)	4708374..4709174	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGJ00_RS22650 (FJMB80023_44260)	4709209..4710105	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T41109 WP_022649837.1 NZ_AP025799:c4705212-4704901 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T41109 NZ_AP025799:c4705212-4704901 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT41109 WP_022649836.1 NZ_AP025799:c4704900-4704610 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT41109 NZ_AP025799:c4704900-4704610 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6