Detailed information of TA system TA041061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4669355..4669957
• Replicon: chromosome
• Accession: NZ_AP025792
• Organism: Enterobacter hormaechei strain FUJ80017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGK49_RS22370
• Protein ID: WP_022649837.1
• Coordinates: 4669646..4669957 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGK49_RS22365
• Protein ID: WP_022649836.1
• Coordinates: 4669355..4669645 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK49_RS22350 (FJMB80017_43980)	4666853..4667755	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGK49_RS22355 (FJMB80017_43990)	4667752..4668387	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGK49_RS22360 (FJMB80017_44000)	4668384..4669313	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGK49_RS22365 (FJMB80017_44010)	4669355..4669645	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGK49_RS22370 (FJMB80017_44020)	4669646..4669957	-	312	WP_022649837.1	hypothetical protein	Toxin
NGK49_RS22375 (FJMB80017_44030)	4670106..4671047	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGK49_RS22380 (FJMB80017_44040)	4671092..4671529	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGK49_RS22385 (FJMB80017_44050)	4671526..4672407	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGK49_RS22390 (FJMB80017_44060)	4672401..4673000	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGK49_RS22395 (FJMB80017_44070)	4673119..4673919	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGK49_RS22400 (FJMB80017_44080)	4673954..4674850	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T41061 WP_022649837.1 NZ_AP025792:c4669957-4669646 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T41061 NZ_AP025792:c4669957-4669646 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT41061 WP_022649836.1 NZ_AP025792:c4669645-4669355 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT41061 NZ_AP025792:c4669645-4669355 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6