Detailed information of TA system TA041013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4645184..4645786
• Replicon: chromosome
• Accession: NZ_AP025784
• Organism: Enterobacter hormaechei strain FUJ80012

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGJ22_RS22105
• Protein ID: WP_022649837.1
• Coordinates: 4645475..4645786 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGJ22_RS22100
• Protein ID: WP_022649836.1
• Coordinates: 4645184..4645474 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGJ22_RS22085 (FJMB80012_43290)	4642682..4643584	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGJ22_RS22090 (FJMB80012_43300)	4643581..4644216	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGJ22_RS22095 (FJMB80012_43310)	4644213..4645142	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGJ22_RS22100 (FJMB80012_43320)	4645184..4645474	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGJ22_RS22105 (FJMB80012_43330)	4645475..4645786	-	312	WP_022649837.1	hypothetical protein	Toxin
NGJ22_RS22110 (FJMB80012_43340)	4645935..4646876	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGJ22_RS22115 (FJMB80012_43350)	4646921..4647358	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGJ22_RS22120 (FJMB80012_43360)	4647355..4648236	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGJ22_RS22125 (FJMB80012_43370)	4648230..4648829	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGJ22_RS22130 (FJMB80012_43380)	4648948..4649748	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGJ22_RS22135 (FJMB80012_43390)	4649783..4650679	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T41013 WP_022649837.1 NZ_AP025784:c4645786-4645475 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T41013 NZ_AP025784:c4645786-4645475 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT41013 WP_022649836.1 NZ_AP025784:c4645474-4645184 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT41013 NZ_AP025784:c4645474-4645184 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6