Detailed information of TA system TA040981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4704114..4704716
• Replicon: chromosome
• Accession: NZ_AP025780
• Organism: Enterobacter hormaechei strain FUJ80008

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGK30_RS22500
• Protein ID: WP_022649837.1
• Coordinates: 4704405..4704716 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGK30_RS22495
• Protein ID: WP_022649836.1
• Coordinates: 4704114..4704404 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK30_RS22480 (FJMB80008_44070)	4701612..4702514	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGK30_RS22485 (FJMB80008_44080)	4702511..4703146	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGK30_RS22490 (FJMB80008_44090)	4703143..4704072	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGK30_RS22495 (FJMB80008_44100)	4704114..4704404	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGK30_RS22500 (FJMB80008_44110)	4704405..4704716	-	312	WP_022649837.1	hypothetical protein	Toxin
NGK30_RS22505 (FJMB80008_44120)	4704865..4705806	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGK30_RS22510 (FJMB80008_44130)	4705851..4706288	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGK30_RS22515 (FJMB80008_44140)	4706285..4707166	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGK30_RS22520 (FJMB80008_44150)	4707160..4707759	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGK30_RS22525 (FJMB80008_44160)	4707878..4708678	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGK30_RS22530 (FJMB80008_44170)	4708713..4709609	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4693546..4704716	11170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T40981 WP_022649837.1 NZ_AP025780:c4704716-4704405 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T40981 NZ_AP025780:c4704716-4704405 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT40981 WP_022649836.1 NZ_AP025780:c4704404-4704114 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT40981 NZ_AP025780:c4704404-4704114 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6