Detailed information of TA system TA040965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4604790..4605392
• Replicon: chromosome
• Accession: NZ_AP025777
• Organism: Enterobacter hormaechei strain FUJ80007

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGJ89_RS21905
• Protein ID: WP_022649837.1
• Coordinates: 4605081..4605392 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGJ89_RS21900
• Protein ID: WP_022649836.1
• Coordinates: 4604790..4605080 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGJ89_RS21885 (FJMB80007_42810)	4602288..4603190	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGJ89_RS21890 (FJMB80007_42820)	4603187..4603822	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGJ89_RS21895 (FJMB80007_42830)	4603819..4604748	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGJ89_RS21900 (FJMB80007_42840)	4604790..4605080	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGJ89_RS21905 (FJMB80007_42850)	4605081..4605392	-	312	WP_022649837.1	hypothetical protein	Toxin
NGJ89_RS21910 (FJMB80007_42860)	4605541..4606482	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGJ89_RS21915 (FJMB80007_42870)	4606527..4606964	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGJ89_RS21920 (FJMB80007_42880)	4606961..4607842	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGJ89_RS21925 (FJMB80007_42890)	4607836..4608435	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGJ89_RS21930 (FJMB80007_42900)	4608554..4609354	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGJ89_RS21935 (FJMB80007_42910)	4609389..4610285	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T40965 WP_022649837.1 NZ_AP025777:c4605392-4605081 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T40965 NZ_AP025777:c4605392-4605081 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT40965 WP_022649836.1 NZ_AP025777:c4605080-4604790 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT40965 NZ_AP025777:c4605080-4604790 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6