Detailed information of TA system TA040933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4665606..4666208
• Replicon: chromosome
• Accession: NZ_AP025772
• Organism: Enterobacter hormaechei strain FUJ80004

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGJ78_RS22325
• Protein ID: WP_022649837.1
• Coordinates: 4665897..4666208 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGJ78_RS22320
• Protein ID: WP_022649836.1
• Coordinates: 4665606..4665896 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGJ78_RS22305 (FJMB80004_43770)	4663104..4664006	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGJ78_RS22310 (FJMB80004_43780)	4664003..4664638	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGJ78_RS22315 (FJMB80004_43790)	4664635..4665564	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGJ78_RS22320 (FJMB80004_43800)	4665606..4665896	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGJ78_RS22325 (FJMB80004_43810)	4665897..4666208	-	312	WP_022649837.1	hypothetical protein	Toxin
NGJ78_RS22330 (FJMB80004_43820)	4666357..4667298	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGJ78_RS22335 (FJMB80004_43830)	4667343..4667780	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGJ78_RS22340 (FJMB80004_43840)	4667777..4668658	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGJ78_RS22345 (FJMB80004_43850)	4668652..4669251	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGJ78_RS22350 (FJMB80004_43860)	4669370..4670170	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGJ78_RS22355 (FJMB80004_43870)	4670205..4671101	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T40933 WP_022649837.1 NZ_AP025772:c4666208-4665897 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T40933 NZ_AP025772:c4666208-4665897 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT40933 WP_022649836.1 NZ_AP025772:c4665896-4665606 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT40933 NZ_AP025772:c4665896-4665606 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6