Detailed information of TA system TA040918

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4639899..4640501
• Replicon: chromosome
• Accession: NZ_AP025770
• Organism: Enterobacter hormaechei strain FUJ80003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGJ61_RS22340
• Protein ID: WP_022649837.1
• Coordinates: 4640190..4640501 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGJ61_RS22335
• Protein ID: WP_022649836.1
• Coordinates: 4639899..4640189 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGJ61_RS22320 (FJMB80003_43820)	4637397..4638299	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGJ61_RS22325 (FJMB80003_43830)	4638296..4638931	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGJ61_RS22330 (FJMB80003_43840)	4638928..4639857	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGJ61_RS22335 (FJMB80003_43850)	4639899..4640189	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGJ61_RS22340 (FJMB80003_43860)	4640190..4640501	-	312	WP_022649837.1	hypothetical protein	Toxin
NGJ61_RS22345 (FJMB80003_43870)	4640650..4641591	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGJ61_RS22350 (FJMB80003_43880)	4641636..4642073	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGJ61_RS22355 (FJMB80003_43890)	4642070..4642951	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGJ61_RS22360 (FJMB80003_43900)	4642945..4643544	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGJ61_RS22365 (FJMB80003_43910)	4643663..4644463	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGJ61_RS22370 (FJMB80003_43920)	4644498..4645394	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T40918 WP_022649837.1 NZ_AP025770:c4640501-4640190 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T40918 NZ_AP025770:c4640501-4640190 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT40918 WP_022649836.1 NZ_AP025770:c4640189-4639899 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT40918 NZ_AP025770:c4640189-4639899 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6