Detailed information of TA system TA040902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4594863..4595465
• Replicon: chromosome
• Accession: NZ_AP025767
• Organism: Enterobacter hormaechei strain FUJ80002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGK40_RS21865
• Protein ID: WP_022649837.1
• Coordinates: 4595154..4595465 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGK40_RS21860
• Protein ID: WP_022649836.1
• Coordinates: 4594863..4595153 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK40_RS21845 (FJMB80002_42670)	4592361..4593263	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGK40_RS21850 (FJMB80002_42680)	4593260..4593895	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGK40_RS21855 (FJMB80002_42690)	4593892..4594821	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGK40_RS21860 (FJMB80002_42700)	4594863..4595153	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGK40_RS21865 (FJMB80002_42710)	4595154..4595465	-	312	WP_022649837.1	hypothetical protein	Toxin
NGK40_RS21870 (FJMB80002_42720)	4595614..4596555	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGK40_RS21875 (FJMB80002_42730)	4596600..4597037	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGK40_RS21880 (FJMB80002_42740)	4597034..4597915	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGK40_RS21885 (FJMB80002_42750)	4597909..4598508	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGK40_RS21890 (FJMB80002_42760)	4598627..4599427	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGK40_RS21895 (FJMB80002_42770)	4599462..4600358	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T40902 WP_022649837.1 NZ_AP025767:c4595465-4595154 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T40902 NZ_AP025767:c4595465-4595154 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT40902 WP_022649836.1 NZ_AP025767:c4595153-4594863 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT40902 NZ_AP025767:c4595153-4594863 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6