Detailed information of TA system TA040888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 30480..31210
• Replicon: chromosome
• Accession: NZ_AP025767
• Organism: Enterobacter hormaechei strain FUJ80002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155SSD8
• Locus tag: NGK40_RS00155
• Protein ID: WP_022646552.1
• Coordinates: 30480..30794 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGK40_RS00160
• Protein ID: WP_032622105.1
• Coordinates: 30794..31210 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK40_RS00130	26146..27231	+	1086	Protein_25	cellulase family glycosylhydrolase	-
NGK40_RS00135 (FJMB80002_00260)	27137..28321	-	1185	WP_022646548.1	multidrug efflux MFS transporter EmrD	-
NGK40_RS00140 (FJMB80002_00270)	28497..29330	-	834	WP_022646549.1	DMT family transporter	-
NGK40_RS00145 (FJMB80002_00280)	29393..29839	-	447	WP_022646550.1	GNAT family N-acetyltransferase	-
NGK40_RS00150	29904..29993	-	90	WP_022646551.1	type I toxin-antitoxin system toxin TisB	-
NGK40_RS00155 (FJMB80002_00300)	30480..30794	+	315	WP_022646552.1	type II toxin-antitoxin system HigB family toxin	Toxin
NGK40_RS00160 (FJMB80002_00310)	30794..31210	+	417	WP_032622105.1	helix-turn-helix domain-containing protein	Antitoxin
NGK40_RS00165	31357..31455	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
NGK40_RS00170 (FJMB80002_00320)	31685..33373	+	1689	WP_022646554.1	acetolactate synthase large subunit	-
NGK40_RS00175 (FJMB80002_00330)	33377..33664	+	288	WP_015570113.1	acetolactate synthase small subunit	-
NGK40_RS00180 (FJMB80002_00340)	33790..34383	+	594	WP_022646555.1	transcriptional regulator UhpA	-
NGK40_RS00185 (FJMB80002_00350)	34380..35885	+	1506	WP_022646556.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.55 Da
• Isoelectric Point: 10.1989

>T40888 WP_022646552.1 NZ_AP025767:30480-30794 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQYREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T40888 NZ_AP025767:30480-30794 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15348.91 Da
• Isoelectric Point: 5.6761

>AT40888 WP_032622105.1 NZ_AP025767:30794-31210 [Enterobacter hormaechei]
MIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVGI
AVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 417 bp

>AT40888 NZ_AP025767:30794-31210 [Enterobacter hormaechei]
ATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGAGAA
AGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTTGCG
CCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGAATT
GCAGTTTTGAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGATGGT
TTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCACCTG
CGCTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A155SSD8