Detailed information of TA system TA040886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4640411..4641013
• Replicon: chromosome
• Accession: NZ_AP025764
• Organism: Enterobacter hormaechei strain FUJ80001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: NGK23_RS22160
• Protein ID: WP_022649837.1
• Coordinates: 4640702..4641013 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NGK23_RS22155
• Protein ID: WP_022649836.1
• Coordinates: 4640411..4640701 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK23_RS22140 (FJMB80001_43290)	4637909..4638811	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
NGK23_RS22145 (FJMB80001_43300)	4638808..4639443	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
NGK23_RS22150 (FJMB80001_43310)	4639440..4640369	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
NGK23_RS22155 (FJMB80001_43320)	4640411..4640701	-	291	WP_022649836.1	NadS family protein	Antitoxin
NGK23_RS22160 (FJMB80001_43330)	4640702..4641013	-	312	WP_022649837.1	hypothetical protein	Toxin
NGK23_RS22165 (FJMB80001_43340)	4641162..4642103	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
NGK23_RS22170 (FJMB80001_43350)	4642148..4642585	-	438	WP_022649839.1	D-aminoacyl-tRNA deacylase	-
NGK23_RS22175 (FJMB80001_43360)	4642582..4643463	-	882	WP_022649840.1	virulence factor BrkB family protein	-
NGK23_RS22180 (FJMB80001_43370)	4643457..4644056	-	600	WP_022649841.1	glucose-1-phosphatase	-
NGK23_RS22185 (FJMB80001_43380)	4644175..4644975	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
NGK23_RS22190 (FJMB80001_43390)	4645010..4645906	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T40886 WP_022649837.1 NZ_AP025764:c4641013-4640702 [Enterobacter hormaechei]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T40886 NZ_AP025764:c4641013-4640702 [Enterobacter hormaechei]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT40886 WP_022649836.1 NZ_AP025764:c4640701-4640411 [Enterobacter hormaechei]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT40886 NZ_AP025764:c4640701-4640411 [Enterobacter hormaechei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6