Detailed information of TA system TA040870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2668509..2669113
• Replicon: chromosome
• Accession: NZ_AP025761
• Organism: Amphritea japonica strain JCM 14782

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7R6PEV1
• Locus tag: PT3_RS12350
• Protein ID: WP_019620268.1
• Coordinates: 2668796..2669113 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PT3_RS12345
• Protein ID: WP_019620269.1
• Coordinates: 2668509..2668799 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PT3_RS12325	2664198..2665001	-	804	WP_019620274.1	tetratricopeptide repeat protein	-
PT3_RS12330	2665194..2665694	-	501	WP_019620273.1	hypothetical protein	-
PT3_RS12335	2666795..2667271	-	477	WP_019620271.1	YHS domain-containing (seleno)protein	-
PT3_RS12340	2667407..2668288	+	882	WP_019620270.1	AraC family transcriptional regulator	-
PT3_RS12345	2668509..2668799	-	291	WP_019620269.1	NadS family protein	Antitoxin
PT3_RS12350	2668796..2669113	-	318	WP_019620268.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PT3_RS12355	2669575..2670489	-	915	WP_156815099.1	DNA-binding response regulator	-
PT3_RS12360	2670523..2673984	-	3462	WP_019620266.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (3-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12091.07 Da
• Isoelectric Point: 9.8875

>T40870 WP_019620268.1 NZ_AP025761:c2669113-2668796 [Amphritea japonica]
MVIVETAVFTKLIKEMLSDDEYKNLQEVLISRPASGDLIKESGGLRKIRWSVQGTGKSGGIRVIYYWVVDDHHIRMLYVY
PKGKQDNLTKAQLAQLKAIVERWQQ

Nucleotide

• Length: 318 bp

>T40870 NZ_AP025761:c2669113-2668796 [Amphritea japonica]
ATGGTTATTGTCGAAACCGCTGTATTCACAAAGCTCATCAAAGAGATGCTGAGTGATGACGAATATAAAAACCTCCAGGA
AGTGCTTATAAGTCGTCCAGCTTCAGGGGATCTGATAAAAGAATCCGGCGGGCTTCGGAAAATCCGCTGGAGTGTACAAG
GGACGGGGAAAAGTGGTGGTATACGCGTAATCTACTATTGGGTAGTTGATGACCATCATATTCGCATGTTGTATGTATAC
CCTAAGGGCAAACAAGACAACTTAACTAAAGCACAATTGGCACAATTGAAAGCCATAGTTGAAAGGTGGCAACAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10982.47 Da
• Isoelectric Point: 5.7643

>AT40870 WP_019620269.1 NZ_AP025761:c2668799-2668509 [Amphritea japonica]
MNNEMFDELLESVQQMDEIVKENKKASRTFDFPDPEVKAIRERLGVSQEKFAFILGVSKRTIENWEQGRRHPTGAARSLL
RVVEADPEHALKALSA

Nucleotide

• Length: 291 bp

>AT40870 NZ_AP025761:c2668799-2668509 [Amphritea japonica]
ATGAATAATGAAATGTTTGATGAGCTTTTAGAAAGCGTTCAGCAGATGGATGAGATTGTAAAAGAAAATAAAAAAGCATC
TCGGACCTTTGATTTTCCCGACCCTGAAGTTAAGGCTATTCGTGAGCGTTTAGGAGTTTCTCAGGAAAAGTTTGCATTCA
TTCTCGGCGTGAGTAAACGCACAATAGAAAACTGGGAGCAAGGGCGTCGACACCCCACGGGAGCAGCGCGATCACTTTTG
CGGGTTGTCGAAGCGGATCCAGAACATGCGCTTAAAGCACTGAGTGCATAA

Structures

Toxin

• AlphaFold DB: A0A7R6PEV1